"Molecular biology concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, and proteins and their biosynthesis, as well as the regulation of these interactions. Researchers in molecular biology use specific techniques native to molecular biology but increasingly combine these with techniques and ideas from genetics and biochemistry. There is not a defined line between these disciplines.
The invention of the polymerase chain reaction (PCR) by K. Mullis and co-workers in 1985 revolutionized molecular biology and molecular medicine. Major research areas, such as biomarker discovery, gene regulation, and cancer research, are challenging today's PCR technologies with more demanding requirements. These include the need for increased throughput, higher assay sensitivity, and reliable data analysis. Assay development and evaluation, reproducibility of data, and time to result are still major problems encountered by researchers.
PCR amplification is performed routinely and thousands of PCR protocols have been developed, yet researchers still encounter technical difficulties with PCR experiments and often fail to obtain specific amplification products. Although there are several different challenges (e.g., smearing, low yield, and nonspecific amplification), there are two main reasons for PCR failure or poor results: the specificity of the reaction and template secondary structure.
This book has been written in easy and fluent language expressive and self-explanatory labelled diagrams, concise but not too brief, written to the point starting from fundamentals and finishing to the most advanced and current concepts. All diagrams have been provided with detailed legends. Some diagrams have been drawn the author himself from the original source, while the remaining ones are quoted from the authentic works."